Rabbit anti-Human CD104/Integrin β4 Monoclonal Antibody for IF/ICC, FC - P16144

Core Product Specifications and Parameters

Biological Background: Integrin beta-4 Function and Localization

• Integrin beta-4 (ITGB4, CD104, GP150) is a 202 kDa transmembrane glycoprotein that pairs with integrin alpha-6 to form a laminin receptor. This complex is critical for hemidesmosome structure and regulates keratinocyte polarity and motility.
• ITGA6:ITGB4 binds to NRG1 (via EGF domain), and this interaction is necessary for NRG1-ERBB signaling. Related references: PMID:20682778
• ITGA6:ITGB4 interacts with IGF1, facilitating IGF1 signaling. Related references: PMID:22351760
• ITGA6:ITGB4 binds to IGF2, required for IGF2 signaling. Related references: PMID:28873464
• Subcellularly, integrin beta-4 localizes to the cell membrane and is highly enriched at hemidesmosomes.
• Predominantly expressed in epithelia; isoforms beta-4D and beta-4E show tissue-specific expression in colon, placenta, epidermis, lung, heart, and other tissues.
• Possesses EGF-like repeats and undergoes phosphorylation and palmitoylation; mutations are associated with epidermolysis bullosa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 611–710 within the extracellular domain of integrin β4, likely presenting an accessible epitope for live-cell or permeabilized staining protocols.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to balance epitope exposure and structural integrity.
• The direct conjugate reduces protocol steps for flow cytometry (FC); titrate the antibody to determine the optimal concentration for your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a single, streamlined source for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This -conjugated anti-CD104/Integrin β4 antibody supports cell adhesion and cancer research applications. Leverage CamelBio's expertise for rare target sourcing and customized conjugation solutions to accelerate your assay development.