Rabbit anti-Human CD104/Integrin β4 Monoclonal Antibody for IF/ICC, FC - P16144

Core Product Specifications and Parameters

Biological Background: Integrin beta-4 Function and Localization

• Integrin beta-4 (ITGB4), also known as GP150, is a member of the integrin beta chain family that pairs with integrin alpha-6 to form a laminin receptor essential for hemidesmosome structure and epithelial cell attachment.
• The alpha-6/beta-4 integrin is critically required for keratinocyte polarity and motility regulation, bridging the extracellular matrix to the intermediate filament cytoskeleton.
• ITGB4 participates in growth factor signaling: ITGA6:ITGB4 binds to NRG1 and is necessary for NRG1-ERBB signaling; similarly, it binds to IGF1 and IGF2, mediating their downstream signaling pathways. Related references: PMID:20682778 PMID:22351760 PMID:28873464
• ITGB4 localizes primarily to the basolateral cell membrane, concentrated at hemidesmosomes in epithelial cells, with minor expression at other adhesion junctions.
• Expression is predominantly epithelial, with isoform beta-4D found in colon and placenta, and isoform beta-4E in epidermis, lung, duodenum, heart, spleen, and stomach.
• Structural keywords highlight EGF-like repeats, disulfide bonds, calcium/magnesium binding, palmitoylation, and phosphorylation; mutations are linked to epidermolysis bullosa.

Experimental Guidance and Technical Tips

• The immunogen (aa 611-710, within the membrane-proximal fibronectin type III repeats) indicates the antibody likely targets an extracellular epitope; for IF/ICC, consider permeabilization protocols to ensure access to this domain.
• For flow cytometry, titrate the antibody to achieve optimal staining indices and include appropriate isotype controls.
• Validate specificity using ITGB4-overexpressing cells or knockout models where feasible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions for diagnostic developers, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-ITGB4 antibody supports research in cell adhesion, epithelial biology, and related disease diagnostics. For customized sourcing of rare targets, our team ensures seamless access to high-quality materials from concept to clinical application.