Rabbit anti-Human CD101/IGSF2 Monoclonal Antibody for IF/ICC, FC - Q93033

Core Product Specifications and Parameters

Biological Background: CD101/IGSF2 Function and Localization

• CD101, also known as immunoglobulin superfamily member 2 (IGSF2), cell surface glycoprotein V7, and EWI-101, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It functions as an inhibitor of T-cell proliferation induced by CD3, suppressing IL2RA expression, IL2 secretion, and tyrosine kinase activity required for IL2 production and cellular proliferation.
• CD101 inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium, while preventing nuclear translocation of NFAT.
• The protein plays a role in suppressing T-cell proliferation through IL10 secretion by cutaneous dendritic cells.
• Subcellular localization is primarily at the plasma membrane, consistent with its function as a cell surface receptor.
• CD101 is expressed in lung, thymus, small intestine, cutaneous dendritic cells, activated T-cells, monocytes, granulocytes, and certain leukemic cells, including CD4+ CD56+ blastic tumor cells and Langerhans cells from LCH patients.
• Key structural features include disulfide bonds, glycosylation, immunoglobulin domains, a signal peptide, and a transmembrane helix.
• The protein may serve as a marker for CD4+ CD56+ leukemic tumor cells.

Experimental Guidance and Technical Tips

• The immunogen (aa 21-410) corresponds to the extracellular region of human CD101, suggesting the antibody recognizes surface-exposed epitopes; for IF/ICC on live cells, permeabilization may not be required, but fixation and permeabilization conditions should be optimized for intracellular detection.
• For flow cytometry (FC), consider using live cell staining protocols with appropriate Fc blocking and isotype controls; antibody titration is recommended to determine the optimal concentration for specific cell populations.
• Validate the antibody in the relevant sample system, such as activated T-cells or CD101-expressing leukemic cell lines, to confirm specificity and performance.
• Due to the conjugation, protect the antibody from light and avoid freeze-thaw cycles; brief centrifugation before use is advisable to remove aggregates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to specialized IVD raw materials like this anti-CD101 monoclonal antibody, ideal for T-cell immunology and oncology applications. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, supporting your project from discovery to clinical development. We bridge the gap between concept and clinic with reliable, high-quality reagents and expert technical consultation.