Rabbit anti-Human CD101/IGSF2 Monoclonal Antibody for IF/ICC and FC - Q93033

Core Product Specifications and Parameters

Biological Background: CD101/IGSF2 Function and Localization

• CD101 (IGSF2, V7, EWI-101) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, characterized by extracellular immunoglobulin-like domains and a cytoplasmic tail.
• It functions as a negative regulator of T-cell activation: inhibits CD3-induced proliferation, downregulates IL2RA (CD25) expression, and reduces IL-2 secretion.
• Mechanistically, CD101 suppresses tyrosine kinases, PLCG1 phosphorylation, and calcium flux, thereby blocking NFAT nuclear translocation essential for T-cell activation.
• The protein is expressed on the plasma membrane of activated T-cells, monocytes, granulocytes, and cutaneous dendritic cells, with tissue presence in lung, thymus, and small intestine.
• CD101 is also detected in some leukemic cells, notably CD4+ CD56+ blastic tumor cells, and in Langerhans cells from Langerhans cell histiocytosis patients.
• UniProt keywords highlight its structural features: signal peptide, disulfide bonds, immunoglobulin domains, transmembrane helix, and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 21-410) covers almost the entire extracellular region, so the antibody likely recognizes the native conformation; it is well suited for detecting cell surface CD101 by IF/ICC and flow cytometry.
• For IF/ICC, try paraformaldehyde fixation with and without permeabilization to optimize membrane-localized staining; surface-only detection may avoid background from intracellular transit forms.
• In flow cytometry, include proper isotype controls and validate on human sample types known to express CD101, such as activated T-cells or dendritic cells.
• While not listed as a validated application, the antibody could theoretically work in western blotting (predicted molecular weight ~115 kDa); any use for WB should be independently verified by the user.
• The antibody is conjugated to , a stable red fluorescent dye; for indirect protocols use an anti-rabbit secondary antibody compatible with the dye's excitation/emission.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, providing technical support and supply-chain solutions from concept to clinic. This CD101/IGSF2 monoclonal antibody serves immunological and oncology research, and we can supply validated antibody pairs, recombinant proteins, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your diagnostic development.