Rabbit anti-Human CD100/SEMA4D Monoclonal Antibody for IF/ICC, FC - Q92854

Core Product Specifications and Parameters

Biological Background: Semaphorin-4D Function and Localization

• Semaphorin-4D (CD100, SEMA4D) is a cell surface receptor and secreted protein belonging to the semaphorin family, with alternative splicing producing multiple isoforms. It plays a pivotal role in cell-cell signaling through interactions with PLXNB1 and PLXNB2 receptors. Related references: PMID:20877282
• In the nervous system, SEMA4D regulates GABAergic synapse development and promotes inhibitory synapse formation in a PLXNB1-dependent manner. It modulates neurite complexity and arborization in hippocampal neurons by activating PLXNB1 and RHOA. Related references: PMID:19788569
• SEMA4D promotes the migration of cerebellar granule cells and induces endothelial cell migration via the PTK2B/PYK2, SRC, and PI3K-AKT pathway. Related references: PMID:16055703
• In the immune system, SEMA4D induces B-cell aggregation and enhances their viability in vitro, highlighting its immunomodulatory functions. Related references: PMID:8876214
• The protein undergoes post-translational modifications including N-glycosylation and phosphorylation, and contains disulfide bonds essential for its structural integrity. Keywords indicate it is a transmembrane glycoprotein, developmental protein, and neurogenesis-related phosphoprotein.
• Subcellularly, SEMA4D is localized at the cell membrane and can be secreted, consistent with its dual role as a surface receptor and soluble ligand.
• Tissues with high expression include skeletal muscle, peripheral blood lymphocytes, spleen, and thymus; lower expression is detected in testes, brain, kidney, small intestine, prostate, heart, placenta, lung, and pancreas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 22-734) of human SEMA4D, suggesting the antibody may recognize both the membrane-bound and secreted forms. For IF/ICC, permeabilization may not be strictly required if targeting surface SEMA4D, but consider mild permeabilization to access intracellular epitopes if needed.
• This conjugate enables direct fluorescence detection, minimizing secondary antibody steps. For flow cytometry, confirm optimal antibody titration and include appropriate isotype controls to ensure specific staining on the target cell population.
• When investigating SEMA4D function in cell signaling or migration assays, validate the antibody reactivity in the relevant human cell lines (e.g., lymphocytes, neuronal cells) under the experimental conditions used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers studying semaphorin signaling, neurobiology, and immuno-oncology, this SEMA4D antibody complements a portfolio that supports cell surface receptor analysis, migration studies, and diagnostic assay development. From concept to clinic, we provide one-stop access to rare target reagents and technical consulting to accelerate your diagnostics pipeline.