Rabbit anti-Human CD100/SEMA4D mAb - Q92854

Core Product Specifications and Parameters

Biological Background: Semaphorin-4D Function and Localization

• Semaphorin-4D (CD100) acts as a cell surface receptor for PLXNB1 and PLXNB2, playing a key role in cell-cell signaling, particularly in neurodevelopment and immune regulation. Related references: PMID:20877282
• It regulates GABAergic synapse development and promotes inhibitory synapse formation in a PLXNB1-dependent manner (By similarity).
• Semaphorin-4D modulates neurite complexity and arborization in hippocampal neurons through PLXNB1-mediated activation of RHOA. Related references: PMID:19788569
• The protein promotes migration of cerebellar granule cells and endothelial cells, with endothelial migration involving the PTK2B/PYK2, SRC, and PI3K-AKT signaling pathway. Related references: PMID:16055703
• In the immune system, semaphorin-4D induces B-cell aggregation and enhances viability in vitro. Related references: PMID:8876214
• Subcellularly, it localizes to the cell membrane and is also secreted; it exhibits strong expression in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus, with lower levels in testes, brain, kidney, and other tissues.
• The protein undergoes glycosylation, phosphorylation, and disulfide bond formation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• This antibody is generated against a recombinant protein spanning the extracellular domain (aa 22-734) of human semaphorin-4D, which may recognize both membrane-bound and secreted forms.
• For immunofluorescence (IF/ICC), fixation with 4% PFA and permeabilization with Triton X-100 are typical starting points; however, optimization for your specific cell type is advised.
• For flow cytometry (FC), titrate the antibody to determine the optimal working dilution for your cell population, using relevant positive (e.g., peripheral blood mononuclear cells) and negative controls.
• Given the expression of CD100 in immune cells and neurons, consider validating the antibody in known positive tissues such as spleen, thymus, or cultured lymphocytes, and in neural cell lines if studying neurodevelopment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing, including ready-to-use anti-CD100 antibody for immunology and neuroscience research. As your one-stop partner, we supply validated monoclonal antibodies, matched antigen controls, and ancillary reagents to streamline assay development from concept to clinic. For rare or customized targets like semaphorin family members, our team ensures reliable procurement and technical support.