Rabbit anti-Human cCD3 mAb for Flow Cytometry (Intracellular) - P07766

Core Product Specifications and Parameters

Biological Background: CD3E Function and Localization

• CD3E is a core subunit of the TCR-CD3 complex on T lymphocytes, playing an indispensable role in adaptive immunity by transmitting activation signals upon antigen recognition. Related references: PMID:15294938 PMID:15546002 PMID:2470098
• The cytoplasmic domain of CD3E contains immunoreceptor tyrosine-based activation motifs (ITAMs) that are phosphorylated by Src-family kinases LCK and FYN upon TCR engagement, leading to ZAP70 recruitment and downstream signaling. Related references: PMID:2470098 PMID:40592325
• CD3E is critically involved in T-cell development, ensuring proper positive and negative selection in the thymus.
• The protein participates in internalization and down-regulation of the TCR-CD3 complex via endocytosis sequences in its cytoplasmic tail, modulating T-cell responsiveness. Related references: PMID:10384095 PMID:26507128
• Beyond TCR signaling, CD3E acts as a receptor for ITPRIPL1, inhibiting T-cell activation by promoting NCK1 binding and blocking ZAP70 interaction, which suppresses ERK-NFkB and calcium flux. Related references: PMID:12110186 PMID:38614099
• CD3E localizes to the plasma membrane and contains a single-pass transmembrane domain and an extracellular immunoglobulin-like domain.
• Its functional roles are underscored by keywords such as adaptive immunity, cell membrane, disulfide bond, phosphorylation, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen targets an intracellular region (amino acids 50–150) of human CD3E, making this antibody appropriate for intracellular flow cytometry after permeabilization.
• Consider using optimized fixation/permeabilization buffers to preserve CD3E epitopes and obtain reliable staining in T-cell samples.
• Validate antibody specificity and sensitivity in relevant T-cell lines (e.g., Jurkat) or primary human peripheral blood T lymphocytes.
• Note that declared reactivity is human; cross-reactivity to other species has not been reported and should be verified independently if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to deliver comprehensive IVD raw-material solutions. This -conjugated anti-CD3E monoclonal antibody targets a key T-cell marker, and our sourcing capabilities extend to matched antibody pairs, validated clones, bulk ancillary reagents, and custom rare-target procurement—supporting your T-cell immunology and IVD assay development from concept to clinic.