Rabbit anti-Human CaSR Monoclonal Antibody for Flow Cytometry - P41180

Core Product Specifications and Parameters

Biological Background: CaSR Function and Localization

• The extracellular calcium-sensing receptor (CaSR), also known as parathyroid cell calcium-sensing receptor 1 (PCaR1), is encoded by the CASR gene and belongs to the G protein-coupled receptor (GPCR) family.
• CaSR senses changes in extracellular calcium concentration and plays a key role in maintaining calcium homeostasis. Activation by elevated circulating calcium leads to decreased parathyroid hormone (PTH) secretion in the parathyroid glands. Related references: PMID:17555508, PMID:19789209, PMID:21566075
• Depending on cellular context, CaSR couples with different G proteins: in the parathyroid and kidney, it signals through G(q)/G(11) and G(i)/G(o), activating phospholipase C-beta and inhibiting adenylate cyclase, respectively. Related references: PMID:38632411, PMID:7759551
• Full receptor activation follows a co-agonist mechanism where aromatic amino acids (e.g., Trp, Phe) act concertedly with divalent cations (e.g., calcium, magnesium). Related references: PMID:27386547, PMID:27434672, PMID:32817431
• CaSR mediates activation of the NLRP3 inflammasome via G(i)/G(o)-mediated down-regulation of cyclic AMP (cAMP). Related references: PMID:32843625
• Subcellular localization is primarily at the cell membrane.
• Tissue specificity includes expression in the brain (temporal lobe, frontal lobe, parietal lobe, hippocampus, cerebellum) and peripheral tissues such as kidney, lung, liver, heart, skeletal muscle, and placenta.
• Post-translational modifications and structural features include glycosylation, phosphorylation, disulfide bond formation, alternative splicing, and ubiquitination, as annotated in UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 211–310 of human CaSR, which lies in the extracellular domain; this design may enhance specificity for native cell surface receptor detection in flow cytometry.
• As an -conjugated monoclonal antibody, it is ready for direct flow cytometry; consider performing titration to optimize staining concentration for your specific cell type and assay conditions.
• The antibody is validated for human reactivity; for other species, examine immunogen sequence homology and experimentally verify cross-reactivity.
• Include appropriate isotype and unstained controls; consider Fc receptor blocking if using cells with high FcR expression to minimize non-specific binding.
• For applications beyond flow cytometry (e.g., immunohistochemistry, western blotting), validate the antibody under your specific experimental conditions, as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop access to IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers from concept to clinic. For researchers focused on calcium signaling, parathyroid hormone regulation, or renal pathophysiology, CamelBio offers validated monoclonal antibodies like this anti-CaSR reagent, along with optimized antibody pairs, bulk ancillary reagents, and sourcing assistance for rare targets. Streamline your procurement and accelerate assay development with CamelBio's integrated supply capabilities.