Rabbit anti-Human CAR/CXADR Monoclonal Antibody for Flow Cytometry - P78310

Core Product Specifications and Parameters

Biological Background: CAR/CXADR Function and Localization

• CAR (Coxsackievirus and Adenovirus Receptor), also known as CXADR, is a transmembrane glycoprotein and member of the immunoglobulin superfamily, functioning as a cell adhesion molecule and receptor for viruses.
• As a component of the epithelial apical junction complex, it mediates homophilic cell adhesion and is essential for tight junction integrity, localizing to tight junctions and adherens junctions at the cell membrane.
• It plays a role in transepithelial migration of leukocytes by interacting with JAML on gamma-delta T cells, triggering PI3K and MAP kinase signaling that promotes epithelial tissue repair.
• Alternatively spliced isoforms exhibit distinct tissue distribution: isoform 1 is found at neuromuscular junctions, isoform 2 in blood vessels, and both at intercalated disks in cardiac muscle; heart expression is elevated in dilated cardiomyopathy.
• It is expressed broadly in pancreas, brain, heart, small intestine, testis, prostate, and at lower levels in liver and lung, with basolateral membrane localization.
• Acts as a host receptor for adenovirus type C and coxsackievirus B1-B6, facilitating viral entry.
• Post-translational modifications include glycosylation, palmitoylation, phosphorylation, and disulfide bond formation, and it may be secreted as a soluble form.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa20-237), suggesting the antibody likely recognizes an epitope in this region; for flow cytometry, ensure the epitope is accessible on live cells.
• Given its role as a cell surface receptor, CAR/CXADR is suitable for surface staining in flow cytometry; include appropriate isotype controls and verify expression in relevant cell types (e.g., epithelial cells).
• For applications beyond FC, such as neutralization studies, empirical validation is recommended as blocking activity is not guaranteed.
• Consider titrating the antibody for optimal staining and include a viability dye to exclude dead cells, as CAR/CXADR may be shed or internalized.
• Because CAR/CXADR is expressed variably across tissues and isoforms, validate target expression in your specific sample system using alternative methods if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, technical services, and consulting for every stage of assay development. This anti-CAR/CXADR antibody supports investigations into viral pathogenesis, cell adhesion, and epithelial barrier function. CamelBio can supply validated antibody pairs, bulk ancillary reagents, and assist with rare target raw-material sourcing to accelerate your diagnostic projects.