Rabbit anti-Human CAR/CXADR mAb - P78310

Core Product Specifications and Parameters

Biological Background: CAR/CXADR Function and Localization

• The coxsackievirus and adenovirus receptor (CAR/CXADR) is a transmembrane protein of the immunoglobulin superfamily, functioning as a component of epithelial apical junction complexes.
• It acts as a homophilic cell adhesion molecule essential for tight junction integrity and facilitates transepithelial migration of leukocytes.
• Interaction with JAML on leukocytes mediates activation of gamma-delta T cells via PI3-kinase and MAP kinase pathways, promoting proliferation, cytokine production, and epithelial repair.
• CAR serves as a host cell receptor for adenovirus type C and coxsackievirus B1 to B6, mediating viral entry.
• Subcellular localization includes the cell membrane (basolateral), tight junctions, adherens junctions, and secreted forms.
• Broadly expressed in pancreas, brain, heart, small intestine, testis, and prostate; lower in liver and lung. Isoforms show tissue-specific distribution (e.g., neuromuscular junctions, intercalated disks).
• Post-translational modifications include glycosylation, palmitoylation, phosphorylation, and disulfide bond formation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 20–237), enabling detection of native cell-surface CAR by flow cytometry.
• For flow cytometry, titrate the antibody in the relevant human cell line or primary cells to determine optimal concentration; use an appropriate isotype control.
• Given its role as a viral receptor, this conjugate may be applied to monitor CAR expression in viral infection studies; validate signal specificity under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials and technical support. This Rabbit anti-Human CAR/CXADR mAb supports research in cell adhesion, viral infection, and immune regulation, and is backed by our comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and associated reagents. We also offer specialized sourcing services to meet your unique assay development needs.