Rabbit anti-Human CAR/CXADR mAb for FC - P78310

Core Product Specifications and Parameters

Biological Background: CAR/CXADR Function and Localization

• CAR/CXADR (Coxsackievirus and adenovirus receptor) is a transmembrane protein belonging to the immunoglobulin superfamily, encoded by the CXADR gene. Multiple isoforms exist due to alternative splicing, with isoform 1 (canonical) being the most characterized.
• It functions as a homophilic cell adhesion molecule and is a critical component of the epithelial apical junction complex, essential for tight junction integrity.
• CXADR mediates transepithelial migration of leukocytes through interaction with JAML on leukocytes, and this interaction activates gamma-delta T-cells, leading to proliferation and cytokine production that promotes epithelial tissue repair.
• It acts as a receptor for adenovirus type C and for coxsackievirus B1 to B6, facilitating viral entry into host cells.
• The protein localizes to the basolateral cell membrane, and is found at tight junctions and adherens junctions; it can also be secreted.
• Tissue expression includes pancreas, brain, heart, small intestine, testis, and prostate; isoform-specific localization is observed in skeletal muscle, cardiac muscle, and blood vessels, with elevated expression in hearts with dilated cardiomyopathy.
• Post-translational modifications include glycosylation, palmitoylation, and phosphorylation; the protein contains a signal peptide, immunoglobulin-like domains, and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 20–237), making this conjugate suitable for live-cell surface staining in flow cytometry.
• Titrate carefully to minimize background and optimize the signal-to-noise ratio; consider using a viability dye to exclude dead cells.
• Validate staining in a known CAR/CXADR-expressing human cell line (e.g., epithelial cells) as a positive control, and include an isotype control.
• For consistent results, avoid fixation or permeabilization that could mask or alter the extracellular epitope recognized by this antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-CAR/CXADR monoclonal antibody exemplifies our ability to supply high-quality reagents for viral receptor and cell adhesion research. Explore our portfolio for validated antibody pairs, bulk ancillary reagents, and expert assistance in sourcing rare-target raw materials to accelerate your diagnostic development.