Rabbit anti-Human Calreticulin Monoclonal Antibody for Flow Cytometry - P27797

Core Product Specifications and Parameters

Biological Background: Calreticulin Function and Localization

• Calreticulin is a calcium-binding chaperone that promotes protein folding and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Related references: PMID:7876246
• It transiently interacts with monoglucosylated glycoproteins to ensure proper folding and assembly. Related references: PMID:7876246
• Calreticulin binds the DNA-binding domain of the glucocorticoid receptor (NR3C1) and mediates its nuclear export. Related references: PMID:11149926
• It is involved in maternal gene expression regulation and may participate in oocyte maturation by regulating calcium homeostasis (By similarity).
• The protein is localized primarily in the endoplasmic reticulum lumen but also found in the cytosol, secreted to the extracellular space, on the cell surface, and in cytoplasmic vesicles.
• Calreticulin undergoes post-translational modifications including acetylation and hydroxylation, contains a disulfide bond, and binds zinc.
• Alternative names include CRP55, Calregulin, ERp60, HACBP, and grp60; its molecular weight is approximately 48 kDa.
• In oocytes, it is stored in cortical granules and exocytosed during the cortical reaction, potentially contributing to the block to polyspermy (By similarity).

Experimental Guidance and Technical Tips

• This antibody targets a C-terminal fragment (aa 335–417) and may be suitable for intracellular flow cytometry after appropriate fixation and permeabilization.
• Optimize staining protocols for your specific cell type; calreticulin’s ER localization requires effective permeabilization to access the epitope.
• Include positive control cells known to express calreticulin, such as human lymphocytes or cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-calreticulin antibody supports research into protein folding, cellular stress, and immune-related disorders. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your assay development and production.