ABflo 488 Rabbit anti-Human C5AR1/CD88 Monoclonal Antibody for Flow Cytometry - P21730

Core Product Specifications and Parameters

Biological Background: C5AR1/CD88 Function and Localization

• C5AR1/CD88 is a member of the G protein-coupled receptor (GPCR) family and functions as the receptor for the anaphylatoxin C5a, a potent chemotactic and inflammatory peptide. Related references: PMID:10636859 PMID:15153520 PMID:1847994
• Upon C5a binding, the receptor stimulates chemotaxis, granule enzyme release, intracellular calcium mobilization, and superoxide anion production, driving innate immune responses. Related references: PMID:10636859 PMID:15153520 PMID:1847994
• Ligand-receptor interaction involves two distinct sites: a high-affinity site on the extracellular N-terminus and a second site within the transmembrane region that triggers downstream signaling events. Related references: PMID:7622471 PMID:8182049 PMID:9553099
• Signaling is primarily mediated through coupling to G(i)/G(o) proteins (GNAI1 or GNAO1), leading to inhibition of adenylate cyclase and modulation of intracellular cAMP levels. Related references: PMID:36806352 PMID:37852260
• Subcellularly, C5AR1 is predominantly localized to the cell membrane, but can also be found in cytoplasmic vesicles upon agonist-induced internalization.
• Post-translational modifications, including glycosylation, phosphorylation, sulfation, and disulfide bond formation, are crucial for proper receptor folding, ligand binding, and signal transduction.

Experimental Guidance and Technical Tips

• The antibody is a rabbit monoclonal conjugated to ABflo 488, enabling direct fluorescence detection in flow cytometry without a secondary antibody. Optimize staining protocols for live-cell or fixed-cell human samples expressing C5AR1.
• Given the immunogen is a synthetic peptide within the N-terminal region (aa 1–100), consider using the same peptide as a blocking control to confirm staining specificity in your experimental setup.
• Human reactivity is confirmed; if cross-reactivity with other species is required, validate independently using appropriate cell lines or primary cells.
• As a GPCR, C5AR1 may undergo rapid internalization after ligand stimulation; to detect surface expression, perform staining at 4°C or in the presence of sodium azide to inhibit receptor turnover.
• Although validated solely for flow cytometry, the antibody may be suitable for other applications (e.g., immunofluorescence, immunoprecipitation) with proper experimental optimization and controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, supplying validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to diagnostic manufacturers and research laboratories worldwide. This ABflo 488 anti-C5AR1/CD88 monoclonal antibody supports immunology and inflammation research, and our integrated supply chain—from antibody development to clinical application—ensures consistent quality and scalability for your project needs.