Rabbit anti-Human BCL6 Monoclonal Antibody for Intracellular Flow Cytometry - P41182

Core Product Specifications and Parameters

Biological Background: BCL6 Function and Localization

• BCL6 (B-cell lymphoma 6) is a transcriptional repressor with a BTB/POZ domain and zinc finger motifs, also known as BCL5, LAZ3, ZBTB27, and ZNF51.
• Primarily required for germinal center (GC) formation and antibody affinity maturation, BCL6 represses genes involved in differentiation, inflammation, apoptosis, and cell cycle control in GC B-cells.
• Functions by binding directly to the DNA sequence 5'-TTCCTAGAA-3' or indirectly by repressing transcription factors, and recruits corepressors and histone deacetylases to silence target genes.
• Allows rapid proliferation of GC B-cells and tolerates DNA breaks during immunoglobulin class switching and somatic hypermutation without inducing p53-dependent apoptosis.
• In follicular helper CD4+ T-cells (TFH), BCL6 promotes TFH gene expression while inhibiting differentiation into TH1, TH2, and TH17 lineages.
• Required for establishment and maintenance of immunological memory for both B- and T-cells.
• Subcellular localization: predominantly nuclear, consistent with its role as a transcription factor.
• Expressed in germinal center T- and B-cells and primary immature dendritic cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 235-350 of human/mouse BCL6, which lies within a region containing critical functional domains. When performing intracellular flow cytometry, consider optimizing fixation and permeabilization conditions to maintain epitope accessibility and fluorophore stability.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions. Include appropriate isotype controls and verify staining in BCL6-expressing cells (e.g., germinal center B-cells) and negative populations.
• Expected to react with human BCL6; cross-reactivity with other species has not been tested. Validate in the relevant sample system for non-human applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing this -conjugated anti-BCL6 antibody alongside a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. With expertise in transcriptional regulation and immunology targets, we support diagnostic manufacturers, laboratories, and research institutes from early development to clinical application. For bulk orders or sourcing of rare targets, contact CamelBio's technical team for tailored solutions.