Rabbit anti-Human BCL6 Monoclonal Antibody for Intracellular Flow Cytometry - P41182

Core Product Specifications and Parameters

Biological Background: BCL6 Function and Localization

• BCL6 is a transcriptional repressor essential for germinal center (GC) formation and antibody affinity maturation, regulating genes involved in differentiation, inflammation, apoptosis, and cell cycle control.
• It binds directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly represses the transcriptional activity of other transcription factors.
• BCL6 allows GC B-cells to proliferate rapidly and tolerate DNA breaks during immunoglobulin class switch recombination and somatic hypermutation without inducing a p53-dependent apoptotic response.
• In follicular helper CD4+ T-cells (T(FH) cells), BCL6 promotes the expression of T(FH)-related genes while inhibiting the differentiation of T(H)1, T(H)2, and T(H)17 cells.
• It also contributes to the establishment and maintenance of immunological memory for both T- and B-cells and suppresses macrophage proliferation by competing with STAT5 for DNA binding.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitin-like conjugation modulate BCL6 activity.
• Subcellular localization is nuclear, where it interacts with corepressors and histone deacetylases to repress target genes.
• Tissue specificity: predominantly expressed in germinal center B- and T-cells and in primary immature dendritic cells.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein encompassing amino acids 235-350 of human BCL6. For intracellular flow cytometry, optimize cell fixation and permeabilization protocols to ensure nuclear antigen accessibility.
• Consider validating the antibody in relevant human germinal center B-cell lines, T(FH) cell models, or primary immune cells, and confirm specificity using BCL6 knockdown or knockout controls if possible.
• Due to its nuclear localization, permeabilization buffers with strong detergents may be required for optimal staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutes with a complete spectrum of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-BCL6 antibody supports intracellular flow cytometry applications for studying germinal center biology and T(FH) cell differentiation. For unique or hard-to-find targets, CamelBio offers tailored sourcing solutions to accelerate your assay development from early concept to clinical readiness.