Rabbit anti-Human BCL6 Monoclonal Antibody for Flow Cytometry (Intracellular) - P41182

Core Product Specifications and Parameters

Biological Background: BCL6 Function and Localization

• BCL6 (B-cell lymphoma 6) is a transcriptional repressor and proto-oncogene, also known as BCL5, LAZ3, ZBTB27, and ZNF51, encoding a zinc-finger and BTB/POZ domain-containing protein.
• It is a master regulator of germinal center (GC) reactions, repressing genes involved in differentiation, inflammation, apoptosis, and cell cycle to permit rapid B-cell proliferation and somatic hypermutation.
• BCL6 binds directly to the specific DNA motif 5'-TTCCTAGAA-3' and also represses transcription factors, indirectly upregulating AICDA by suppressing microRNAs such as miR-155.
• In follicular helper T-cells (TFH), BCL6 promotes TFH-specific gene expression while inhibiting differentiation into TH1, TH2, and TH17 lineages, and is essential for immunological memory in both T- and B-cells.
• Beyond lymphocytes, BCL6 suppresses macrophage proliferation by competing with STAT5 for binding motifs on target genes like CCL2 and CCND2.
• BCL6 localizes predominantly to the nucleus, consistent with its function as a DNA-binding transcriptional regulator.
• It is expressed in germinal center T- and B-cells and primary immature dendritic cells, with aberrant expression implicated in B-cell lymphomas via chromosomal rearrangements.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which modulate its stability, repressor activity, and interaction with corepressors.

Experimental Guidance and Technical Tips

• The immunogen (residues 235–350 of human/mouse BCL6) corresponds to a central region containing functionally important domains; epitope accessibility in intracellular staining may depend on effective nuclear permeabilization.
• For flow cytometry, use fixation and permeabilization protocols validated for nuclear proteins, and include an appropriate isotype control as well as known positive (e.g., Ramos, Daudi B-cell lines) and negative cell populations.
• Antibody concentration should be optimized by titration; the provided application is intracellular flow cytometry—other applications have not been tested and require individual validation.
• BCL6 expression can be transient and context-dependent; consider stimulation or differentiation conditions to modulate expression levels in experimental systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for B-cell lymphoma and immunology research. Leveraging deep expertise in rare target sourcing, we offer this anti-BCL6 antibody as part of our one-stop procurement solution, streamlining your path from early-stage development to clinical application.