Rabbit anti-Human B7-H2/CD275 Monoclonal Antibody for Flow Cytometry - O75144

Core Product Specifications and Parameters

Biological Background: B7-H2/CD275 (ICOS Ligand) Function and Localization

• ICOSLG encodes B7-H2 (CD275), a member of the immunoglobulin superfamily and the B7 family of immune regulatory proteins; alternative splicing generates multiple isoforms.
• Functions as a ligand for the T-cell-specific receptor ICOS, providing a costimulatory signal for T-cell proliferation and cytokine secretion. Related references: PMID:11007762 PMID:11023515 PMID:30498080
• Also induces B-cell proliferation and differentiation into plasma cells, contributing to adaptive immunity.
• Mediates local tissue responses to inflammatory conditions and modulates secondary immune responses by costimulating memory T-cell function (By similarity).
• In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants such as CXCL8/IL8 and N-formyl-methionyl peptides. Related references: PMID:30498080
• Expressed on the cell membrane of peripheral blood B-cells, monocytes, monocyte-derived dendritic cells, and widely in lymphoid and non-lymphoid tissues (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus, tonsil).
• Features immunoglobulin-like domains, disulfide bonds, and glycosylation; associated with keywords: adaptive immunity, B-cell activation, glycoprotein, signal, transmembrane.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 19–256), suggesting the antibody likely recognizes the native conformation on live cells, making it suitable for flow cytometry.
• As a direct conjugate, this antibody simplifies staining protocols by eliminating the need for a secondary antibody; titrate to determine the optimal concentration for your cell system.
• Verify performance using human B-cell lines or activated monocytes as positive controls, and resting T cells as a negative control; stimulation with PMA/ionomycin or CD40L may upregulate B7-H2 expression.
• Reported cross-reactivity is human; if working with other species, validate specificity before use.
• For intracellular staining, consider that the epitope resides in the extracellular portion; appropriate permeabilization methods may be required if targeting internal pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including this anti-B7-H2/CD275 antibody for immune checkpoint research. Beyond this monoclonal antibody, CamelBio supplies validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets across the B7 family, supporting the full development workflow from concept to clinic.