Rabbit anti-Human AXL Monoclonal Antibody for Flow Cytometry - P30530

Core Product Specifications and Parameters

Biological Background: AXL Function and Localization

• AXL, encoded by the AXL (UFO) gene, is a receptor tyrosine kinase belonging to the TAM family (Tyro3, AXL, Mer) and is also known as ARK, UFO, JTK11, and Tyro7.
• AXL transduces extracellular signals by binding its ligand GAS6, regulating cell survival, proliferation, migration, and differentiation. Ligand binding induces dimerization and autophosphorylation, activating downstream AKT signaling via PI3-kinase and GRB2.
• AXL localizes at the cell membrane as a single-pass type I membrane protein.
• AXL inhibits Toll-like receptor (TLR)-mediated innate immune signaling, modulating the immune response.
• AXL serves as a host cell receptor for multiple viruses, including Lassa virus, lymphocytic choriomeningitis virus, Ebolavirus, and Zika virus. For Zika virus, AXL promotes entry in glial cells, Sertoli cells, and astrocytes and potentiates kinase activity to antagonize type I interferon signaling via a SOCS1-dependent mechanism. Related references: PMID:28076778 PMID:29379210 PMID:31311882
• AXL is highly expressed in metastatic colon tumors, with lower expression in primary tumors and weak expression in normal colon tissue, suggesting a role in cancer progression.
• Key post-translational modifications of AXL include glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, consistent with its signaling and turnover functions.

Experimental Guidance and Technical Tips

• The immunogen (aa 33-449) encompasses the extracellular domain of human AXL, suggesting the antibody likely recognizes the native cell surface receptor, making it suitable for flow cytometry.
• For flow cytometry, titrate the antibody to determine optimal staining concentration, employ viability dyes to exclude dead cells, and include appropriate isotype control antibodies for accurate gating.
• Validate antibody specificity on positive controls such as AXL-transfected cells or known AXL-expressing cell lines, along with negative controls.
• As a directly conjugated antibody (ABflo 488), secondary detection is unnecessary; protect from light during staining and acquisition to maintain signal integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic sourcing partner for IVD manufacturers and research laboratories, offering reliable access to raw materials including this ABflo 488-conjugated anti-AXL antibody. With expertise in immunological reagents, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support rare target sourcing to accelerate your diagnostic or research workflows.