Rabbit anti-Human AXL Monoclonal Antibody for FC - P30530

Core Product Specifications and Parameters

Biological Background: AXL Function and Localization

• AXL (UFO, ARK, Tyro7, JTK11) is a receptor tyrosine kinase encoded by the AXL gene, featuring extracellular immunoglobulin-like domains, a transmembrane helix, and an intracellular kinase domain.
• Upon binding its ligand GAS6, AXL dimerizes and autophosphorylates, triggering downstream signaling via PI3K/AKT, GRB2, and other effectors to regulate cell survival, proliferation, migration, and differentiation.
• Highly expressed in metastatic colon tumors and weakly in normal colon tissue, implicating AXL in cancer progression and metastasis.
• Functions as a host cell receptor for multiple viruses, including lassa virus, lymphocytic choriomeningitis virus, Ebolavirus, and Zika virus, often via GAS6-mediated recognition of phosphatidylserine on the viral envelope. Related references: PMID:28076778 PMID:29379210 PMID:31311882
• Subcellularly localizes primarily to the cell membrane as a single‑pass type I membrane protein.
• Classified as an oncogene and proto‑oncogene with ATP‑binding, immunoglobulin domain, glycoprotein, and alternative splicing features; key in innate immunity and viral entry.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 33‑449), which encompasses the ligand‑binding domain; thus the antibody likely recognizes the native cell‑surface receptor.
• This ‑conjugated antibody is optimized for direct flow cytometry, eliminating the need for a secondary detection reagent.
• For flow cytometry, a typical starting dilution range is 1:50–1:200, but optimal working concentration must be determined empirically in the relevant cell system.
• While not validated for other applications, researchers may consider using this antibody in assays where the extracellular domain is accessible, such as cell‑based ELISA, with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw‑material supply chain solutions, offering validated monoclonal antibodies like this anti‑AXL product, along with optimized antibody pairs, bulk ancillary reagents, and access to rare target raw materials. Whether your research focuses on cancer biomarker development or viral infection diagnostics, CamelBio streamlines sourcing from concept to clinic.