Rabbit anti-Human AXL mAb for FC - P30530

Core Product Specifications and Parameters

Biological Background: AXL Function and Localization

• AXL, also known as UFO, ARK, JTK11, and Tyro7, is a receptor tyrosine kinase encoded by the AXL gene (GeneID 558).
• It transduces signals by binding the ligand GAS6, regulating cell survival, proliferation, migration, and differentiation.
• Upon ligand binding, AXL dimerizes, autophosphorylates, and activates multiple downstream signaling pathways, including PI3K-AKT, via phosphorylation of PIK3R1, GRB2, and others.
• AXL is located on the cell membrane and is highly expressed in metastatic colon tumors, but weakly in normal colon tissue.
• It plays a role in innate immunity by inhibiting Toll-like receptor (TLR)-mediated responses.
• AXL serves as a host cell receptor for multiple viruses, including Lassa virus, lymphocytic choriomeningitis virus, and Ebola virus, likely via GAS6 bridging to phosphatidylserine on virions.
• Zika virus utilizes AXL for entry into glial cells, Sertoli cells, and astrocytes, and potentiates AXL kinase activity to antagonize type I interferon signaling via SOCS1, promoting infection. Related references: PMID:28076778, PMID:29379210, PMID:31311882
• Post-translational modifications include phosphorylation, glycosylation, disulfide bond formation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 33-449) of human AXL; this design is well-suited for detecting native surface receptor on live cells by flow cytometry.
• Consider validating the antibody in a relevant human cell line with known AXL expression (e.g., metastatic colon cancer cells) and using proper isotype controls.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration and use a viability dye to exclude dead cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to premium IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-AXL antibody supports oncology and viral entry research, and our team can assist with custom sourcing for rare targets or conjugate formats. Partner with CamelBio to accelerate your assay development from concept to clinic.