Rabbit Anti-HPCAL1 Polyclonal Antibody for WB and ELISA - P37235

Core Product Specifications and Parameters

Biological Background: HPCAL1 Function and Localization

• HPCAL1, also known as Hippocalcin-like protein 1, calcium-binding protein BDR-1, HLP2, or Visinin-like protein 3 (VILIP-3), belongs to the neuronal calcium sensor (NCS) family of EF-hand calcium-binding proteins.
• The protein contains multiple functional EF-hand motifs and an N-terminal myristoylation consensus sequence, characteristic of calcium-dependent membrane-associating proteins (Keywords: Calcium, Lipoprotein, Myristate, Repeat).
• HPCAL1 may be involved in the calcium-dependent regulation of rhodopsin phosphorylation, implicating it in phototransduction or related G-protein coupled receptor signaling pathways.
• Subcellularly, HPCAL1 is membrane-associated, likely mediated by its N-terminal myristoyl group in a calcium-dependent manner (SUBCELLULAR LOCATION: Membrane).
• Post-translational N-myristoylation is essential for its proper membrane targeting and function, as indicated by the Myristate keyword.
• The protein has been structurally characterized (3D-structure keyword), revealing calcium-induced conformational changes typical of NCS proteins.
• HPCAL1 is included in the human reference proteome and has been identified through large-scale proteomics studies (Proteomics identification keyword).

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human HPCAL1 protein (residues 1-193), fused to a recombinant tag. For Western blotting, validate specificity by comparing signals between HPCAL1 knockout and wild-type cell or tissue lysates.
• In WB, expect a band at approximately 22 kDa. Always include appropriate molecular weight markers and loading controls to confirm protein identity.
• For ELISA, optimize coating antigen and antibody concentrations through titration. If cross-reactivity with related visinin-like proteins is a concern, consider pre-adsorption or competitive assays.
• Although this antibody is KO-validated by the supplier, we recommend independent validation in your specific experimental system to confirm performance and suitability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers and research labs reliable antibodies, antigens, and ancillary reagents. This anti-HPCAL1 polyclonal antibody supports neuroscience and calcium signaling research, with applications in WB and ELISA. Beyond catalog products, we provide custom antibody development, bulk supply, and rare-target sourcing to streamline your entire development pipeline from concept to clinic.