Rabbit Anti-HNF4a Polyclonal Antibody for WB, ELISA – HNF4a

Core Product Specifications and Parameters

Experimental Guidance and Technical Tips

• For Western blot, optimize primary antibody dilution and use positive (e.g., HepG2 or mouse liver lysate) and negative controls to verify specificity.
• The antibody was raised against a peptide within amino acids 100–200 of human HNF4a; sequence alignment suggests high homology with mouse, explaining the cross-reactivity.
• In ELISA, the immunogen peptide can serve as a coating antigen to establish assay conditions and check antibody activity.
• The predicted molecular weight is 51–52 kDa; be aware of potential isoforms or post-translational modifications that may alter migration.
• For sandwich ELISA, this polyclonal antibody may be used as a detection antibody; however, pairing with a suitable capture antibody requires experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with premium raw materials like this anti-HNF4a polyclonal antibody, supporting studies in hepatocyte biology and metabolic disorders. Our portfolio includes validated antibody pairs, optimized monoclonals and polyclonals, and bulk ancillary reagents for efficient assay development. Leverage our sourcing network to find rare-target raw materials and accelerate your diagnostic pipeline from concept to clinic.