Rabbit Anti-GNAI3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P08754

Core Product Specifications and Parameters

Biological Background: GNAI3 Function and Localization

• GNAI3 is a member of the G(i) alpha subunit family, functioning as a molecular switch in GPCR signaling. It cycles between GDP-bound inactive and GTP-bound active states.
• The GTPase activity of GNAI3 is modulated by regulatory proteins that accelerate both GDP release and GTP hydrolysis. Related references: PMID:18434541 PMID:19478087 PMID:8774883
• In its active state, GNAI3 inhibits adenylate cyclase to reduce cAMP levels and stimulates receptor-regulated potassium channels. Related references: PMID:19478087 PMID:2535845
• GNAI3 plays a role in cell division by regulating the centrosomal localization of RGS14; the active GTP-bound form prevents RGS14 association with centrosomes. Related references: PMID:17635935
• Additionally, the GTP-bound GNAI3 can activate the calcium-permeable channel TRPC5. Related references: PMID:37137991
• Subcellularly, GNAI3 is found in the cytoplasm, associated with the cell membrane, and at the centrosome.
• As a lipid-anchored protein, GNAI3 undergoes myristoylation and palmitoylation, which are critical for its membrane localization and signaling function. It is also a target for ADP-ribosylation by bacterial toxins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human GNAI3 protein (amino acids 1–354), suggesting the antibody may recognize multiple epitopes. Consider validating reactivity in human and mouse samples, as cross-reactivity is reported.
• For Western blot, GNAI3 is expected at ~41 kDa. Use appropriate loading controls and optimize antibody dilution.
• In immunofluorescence/immunocytochemistry, expect cytoplasmic, membrane, and possibly centrosomal staining. Permeabilization may be required for intracellular detection.
• For immunoprecipitation, the polyclonal nature may aid in capturing native protein complexes.
• ELISA may be used for quantitative detection; consider pairing with a GNAI3 standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. Our GNAI3 antibody supports research in GPCR signaling, cell cycle regulation, and channel biology. Whether you need optimized monoclonal/polyclonal antibodies or customized raw-material supply, CamelBio bridges the gap from concept to clinic with one-stop efficiency.