Rabbit Anti-GCA Polyclonal Antibody for WB, IF/ICC, ELISA - P28676

Core Product Specifications and Parameters

Biological Background: GCA Function and Localization

• Grancalcin (GCA, alias GCL) is a member of the penta-EF-hand family of calcium-binding proteins.
• It functions in neutrophil adhesion to fibronectin and may contribute to focal adhesion formation.
• Subcellularly, GCA localizes to the cytoplasm and can associate with cytoplasmic granule membranes.
• Highly expressed in bone marrow; detected at the protein level in neutrophils and macrophages.
• Contains EF-hand domains that mediate calcium binding, as indicated by keywords including Calcium, Metal-binding, and Repeat.
• UniProt lists a calculated molecular weight of 24,010 Da, consistent with the observed 24 kDa band.
• Structural studies have been performed (3D-structure keyword); protein identity confirmed by direct protein sequencing and proteomics.

Experimental Guidance and Technical Tips

• The immunogen encompasses nearly the full-length GCA protein (aa 1-217), suggesting the antibody may recognize multiple epitopes and is suitable for detecting native and denatured protein.
• For Western blot (WB), consider a predicted band size of approximately 24 kDa; use appropriate positive controls such as bone marrow, neutrophil, or macrophage lysates.
• For immunofluorescence (IF/ICC), validate subcellular localization in cell types expressing GCA (e.g., neutrophils, macrophages); permeabilization may be required to detect cytoplasmic signal.
• When using ELISA, the antibody as a polyclonal can be employed as capture or detection reagent; validate with recombinant GCA protein or cell lysates from GCA-expressing tissues.
• Since mouse cross-reactivity is reported, this antibody may be useful for studies in mouse models; however, verify specificity in the relevant mouse tissue or cell line before use.

CamelBio: Your One-Stop Sourcing Bridge

For research into granulocyte function and adhesion, CamelBio provides comprehensive IVD raw material solutions. We supply this anti-GCA polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our team supports diagnostic manufacturers, laboratories, and research institutes from concept to clinical application.