Rabbit Anti-GAP43 Polyclonal Antibody for WB, IHC-P, ELISA - P17677

Core Product Specifications and Parameters

Biological Background: GAP43 Function and Localization

• GAP43, also known as neuromodulin, B-50, pp46, and growth-associated protein 43, is a neural-specific protein encoded by the GAP43 gene.
• It is a major component of motile growth cones at the tips of elongating axons, playing a critical role in axonal and dendritic filopodia induction.
• The protein localizes to the cytoplasmic face of the plasma membrane and is enriched in growth cone membranes, filopodia, axons, dendrites, and synaptic compartments.
• GAP43 is a calmodulin-binding protein, and its function is regulated by phosphorylation and palmitoylation.
• It is classified as a developmental protein with roles in neurogenesis, differentiation, and growth regulation.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human GAP43 protein (aa 1–238), so the antibody may recognize both native and denatured protein forms.
• For western blot (WB), consider loading 20–30 µg of total protein lysate from neural tissue or cell lines; expect a band near 25 kDa.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval with citrate buffer (pH 6.0) may be required; validate staining in brain or nerve tissue.
• Cross-reactivity with mouse and rat has been confirmed; the antibody is suitable for studies in these species.
• For ELISA, the recombinant immunogen can be used as the coating antigen; titer optimization is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For GAP43-related neurobiology and neurodegenerative disease research, we offer reliable sourcing of high-quality antibodies and customized support. Whether you need standard catalog products or assistance with rare target material procurement, CamelBio bridges the gap from concept to clinic.