Rabbit Anti-FREM1 Polyclonal Antibody for WB, ELISA - Q5H8C1

Core Product Specifications and Parameters

Biological Background: FREM1 Function and Localization

• FREM1 (FRAS1-related extracellular matrix protein 1), also known as QBRICK, is a large secreted glycoprotein encoded by the FREM1 gene (aliases: BNAR, MOTA, TILRR, TRIGNO2, C9orf143, C9orf145, C9orf154) in human chromosomal region 9q22.
• It functions as an extracellular matrix (ECM) protein that is crucial for epidermal differentiation and for maintaining epidermal adhesion during embryonic development.
• FREM1 is a member of the FRAS1/FREM family of ECM proteins, which play key roles in basement membrane integrity and epithelial-mesenchymal interactions.
• The protein localizes to the basement membrane and is secreted into the extracellular space, consistent with its role in ECM organization and cell adhesion.
• Structural features include multiple domains such as calcium-binding lectin-like repeats, disulfide bonds, and glycosylation sites, which are important for its ECM scaffolding function.
• Alternative splicing generates multiple isoforms of FREM1, which may have distinct tissue distributions or functional properties.
• Mutations in FREM1 are associated with human diseases such as craniosynostosis, highlighting its critical role in developmental processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a specific internal region (aa 1880–2179) of human FREM1; this design may allow detection of multiple isoforms containing this segment, but consider the possibility of isoform-specific expression patterns.
• For Western blot, note the large predicted molecular weight of 244 kDa; using low-percentage SDS-PAGE gels and prolonged transfer times may improve detection efficiency.
• The listed cross-reactivity with human, mouse, and rat suggests sequence conservation in the immunogen region; verify reactivity in the intended species’ biological context if not previously tested.
• In ELISA applications, consider using the recombinant immunogen fragment or full-length protein as a positive control to establish assay sensitivity and specificity.
• As a polyclonal antibody, recognize that it comprises a heterogeneous population of target-binding immunoglobulins; lot-to-lot consistency can be influenced by immunization and purification protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and researchers with streamlined access to critical reagents throughout development. Our portfolio, featuring validated FREM1-targeting antibodies like this polyclonal, supports studies in epidermal biology, extracellular matrix research, and developmental disorders. Beyond antibodies, we offer optimized antibody pairs, bulk ancillary reagents, and specialized sourcing for rare or custom targets to accelerate your project from concept to clinic.