Rabbit anti-Dog CD8A Monoclonal Antibody for Flow Cytometry - P33706

Core Product Specifications and Parameters

Biological Background: CD8A Function and Localization

• T-cell surface glycoprotein CD8 alpha chain, encoded by the CD8A gene, is a single-pass type I membrane protein that functions as a coreceptor in adaptive immunity.
• In T-cells, CD8A interacts with the T-cell receptor (TCR) and MHC class I:peptide complexes on antigen-presenting cells, recruiting the Src kinase LCK to initiate intracellular signaling cascades.
• This signaling leads to lymphokine production, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), enabling recognition and elimination of infected cells and tumor cells.
• In natural killer (NK) cells, CD8A homodimers at the cell surface promote a survival mechanism that facilitates conjugation and lysis of multiple target cells.
• CD8A homodimers also support the survival and differentiation of activated lymphocytes into memory CD8 T-cells, contributing to long-term immune memory.
• The protein is localized to the cell membrane and contains an immunoglobulin domain, transmembrane helix, and features such as disulfide bonds, glycosylation, and palmitoylation that are critical for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25–139, corresponding to the extracellular region of dog CD8A; thus, the antibody is expected to recognize an extracellular epitope on live cells.
• For flow cytometry, consider staining live dog peripheral blood mononuclear cells (PBMCs) or isolated T‑cell populations, using appropriate isotype controls and a viability dye.
• Titrate the antibody to determine the optimal concentration for specific staining in your experimental system, and include Fc receptor blocking to reduce non‑specific binding.
• This conjugate is designed for direct detection; no secondary antibody is needed, but ensure instrument compatibility with the far‑red fluorophore.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams by supplying validated IVD‑grade antibodies and recombinant proteins. This anti‑dog CD8A monoclonal antibody facilitates flow‑cytometric analysis of T‑cell subsets in immunology and veterinary diagnostics. Beyond individual reagents, we offer tailored sourcing for rare‑target raw materials, including antibody pairs, bulk ancillary reagents, and custom development assistance to accelerate your project from concept to clinic.