Rabbit Anti-CDC42 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P60953

Core Product Specifications and Parameters

Biological Background: CDC42 Function and Localization

• CDC42 (Cell division control protein 42 homolog), also known as G25K GTP-binding protein, is a small GTPase of the Rho family that cycles between an active GTP-bound and inactive GDP-bound state.
• It regulates multiple cellular processes including epithelial cell polarization, bipolar attachment of spindle microtubules to kinetochores during metaphase, and cell migration. Related references: PMID:15642749 PMID:17038317 PMID:22843693
• In neurons, CDC42 is required for the formation and maintenance of filopodia, thin actin-rich surface projections, and modulates dendritic spine structural plasticity upon CaMKII activation (By similarity). Related references: PMID:14978216
• It localizes to the cell membrane, cytoplasm, cytoskeleton (including microtubule-organizing center, spindle, midbody), cell projections (dendrites), and secretory vesicles such as the acrosome.
• CDC42 organizes the F-actin cytoskeleton during phagocytic cup formation and, upon activation by PLEKHG4B, drives actin remodeling at epithelial cell-cell junctions. Related references: PMID:26465210 PMID:33310911
• Post-translational modifications, including prenylation, methylation, and phosphorylation, regulate its membrane association and activity; the protein is also subject to ubiquitination.
• It negatively regulates SEPTIN12 polymerization, implicating CDC42 in spermatogenesis. Related references: PMID:30189608
• Mutations in CDC42 are associated with intellectual disability and other disease variants, highlighting its clinical importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 118–191 of human CDC42, a region conserved across human, mouse, and rat; this aligns with the validated cross-reactivity and suggests utility in multi-species studies.
• For western blotting, the predicted molecular weight is approximately 21 kDa; consider using reducing conditions and appropriate loading controls.
• In immunohistochemistry (IHC-P), antigen retrieval steps may be necessary; optimal primary antibody concentration should be determined experimentally.
• Immunofluorescence/ICC applications may require cell permeabilization; validate staining specificity in your cellular system.
• For ELISA, confirm suitable antibody pair compatibility and detection limits through empirical testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to support research and diagnostic development. In the area of cell signaling and cytoskeletal dynamics, we help you secure anti-CDC42 antibodies and related tools, complemented by technical services and consulting to accelerate your project from concept to clinic.