Rabbit Anti-CD62E/E-Selectin Monoclonal Antibody for WB, FC, ELISA - P16581

Core Product Specifications and Parameters

Biological Background: E-Selectin Function and Localization

• E-selectin, also known as CD62E, ELAM-1, or LECAM2, is a cell-surface glycoprotein encoded by the SELE gene.
• It belongs to the selectin family of adhesion molecules and mediates leukocyte adhesion to cytokine-activated endothelium via interaction with SELPLG/PSGL1.
• This transmembrane protein features an N-terminal lectin domain, an EGF-like domain, multiple sushi (SCR) repeats, and a short cytoplasmic tail, classifying it as a calcium-dependent (C-type) lectin.
• Subcellularly, E-selectin is localized to the cell membrane, and its expression is induced on endothelial cells upon activation by inflammatory cytokines.
• Post-translational modifications include glycosylation and disulfide bond formation, which are critical for its lectin domain function and overall stability.
• Beyond adhesion, E-selectin may participate in capillary morphogenesis, contributing to angiogenesis.
• The native protein has a predicted molecular weight of approximately 66.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (residues 22–230) of human E-selectin, which includes the lectin and part of the EGF-like domain. For Western blot, a band near 66 kDa is anticipated; reducing and non-reducing conditions may influence migration due to disulfide bonds.
• As a rabbit monoclonal antibody, it offers consistent performance. We recommend validating the antibody in your specific sample and application system.
• For flow cytometry, use live or fixed/permeabilized human endothelial or transfected cells. Include an appropriate isotype control.
• In ELISA, the antibody can be paired with a compatible detection antibody or used directly as a capture reagent. Optimal dilutions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing platform for IVD raw materials, supporting diagnostic developers from research to commercialization. This anti-CD62E antibody facilitates studies in inflammation, immune trafficking, and vascular biology, complementing CamelBio's portfolio of validated antibodies, matched pairs, and bulk ancillary reagents. For your specialized raw material needs—including rare targets and custom development—CamelBio serves as a reliable sourcing partner.