Rabbit anti-CD14 Monoclonal Antibody for Flow Cytometry - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14 is a monocyte differentiation antigen (My23 antigen, myeloid cell-specific leucine-rich glycoprotein) that serves as a coreceptor for bacterial lipopolysaccharide (LPS). Related references: PMID:1698311, PMID:23264655
• In concert with LBP, CD14 binds monomeric LPS and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial LPS. Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Related references: PMID:20133493, PMID:22265692, PMID:23264655, PMID:8612135
• Functions as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides; these signaling clusters are targeted to the Golgi in a lipid-raft dependent pathway. Related references: PMID:16880211
• Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). Related references: PMID:23880187
• Subcellular localization includes the cell membrane, membrane rafts, and Golgi apparatus; a secreted form also exists.
• Tissue specificity: expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
• Keywords: GPI-anchor, leucine-rich repeat, glycoprotein, innate immunity, inflammatory response.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 20–352), suggesting the antibody likely recognizes an extracellular epitope suitable for staining live, non-permeabilized cells in flow cytometry.
• Consider direct immunofluorescence staining of human peripheral blood mononuclear cells (PBMCs) or cynomolgus leukocyte preparations; titrate the antibody to determine the optimal working concentration.
• Validate performance in the relevant sample system and include appropriate isotype controls and fluorescence compensation when using conjugate.
• Cross-reactivity with cynomolgus CD14 enables translational studies in non-human primate models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-CD14 antibody supports innate immunity and monocyte/macrophage research, streamlining your development workflow. Partner with CamelBio to bridge the gap from concept to clinic with reliable raw-material supply and technical expertise.