Rabbit Anti-CC2D1A Polyclonal Antibody for WB, IHC-P, ELISA - Q6P1N0

Core Product Specifications and Parameters

Biological Background: CC2D1A Function and Localization

• CC2D1A (Coiled-coil and C2 domain-containing protein 1A) is a transcription factor that binds the dual repressor element (DRE) and represses HTR1A gene transcription in neuronal cells, a regulation linked to anxiety and major depression.
• It mediates HDAC-independent repression of the HTR1A promoter, and its DNA-binding activity is specifically inactivated by calcium and ATP.
• The protein is essential for functional maturation of synapses (by similarity) and plays distinct roles depending on its subcellular localization.
• In the cytoplasm, CC2D1A acts as a scaffold protein in the PI3K/PDK1/AKT signaling pathway; in the nucleus, it represses HTR1A.
• At the centrosome, CC2D1A regulates spindle pole localization of the cohesin subunit SCC1/RAD21, mediating centriole cohesion during mitosis.
• Also known as Freud-1, Aki1, and Lgd2, it is implicated in intellectual disability and features alternative splicing, coiled-coil domains, and phosphoprotein motifs.
• Subcellular localization includes the cytoplasm, nucleus, and centrosome, consistent with its diverse functional roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-150) of human CC2D1A; users should validate the antibody in their specific sample system for the intended application (WB, IHC-P, or ELISA).
• For Western blot, consider titrating the antibody to determine optimal working concentration in lysates from human, mouse, or rat tissues.
• In IHC-P, antigen retrieval may be necessary; optimization of fixation and permeabilization protocols is recommended.
• As a polyclonal antibody, it may detect multiple isoforms or degradation products; confirm band specificity with appropriate controls.
• Cross-reactivity with mouse and rat has been reported, making it suitable for rodent models of neurological and cancer research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For neuroscience and gene regulation research, we supply high-quality antibodies like this anti-CC2D1A polyclonal antibody, along with validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your diagnostic and research workflows.