Rabbit anti-Cat CD8 alpha Monoclonal Antibody for Flow Cytometry - P41688

Core Product Specifications and Parameters

Biological Background: CD8 Alpha Function and Localization

• CD8 alpha is a T-cell surface glycoprotein that serves as a coreceptor for MHC class I molecules, recognizing peptide-MHC class I complexes and facilitating T-cell receptor (TCR) signaling.
• Upon binding to the appropriate ligand, CD8 alpha recruits the Src kinase LCK to the TCR-CD3 complex, initiating phosphorylation cascades that drive cytotoxic T-lymphocyte (CTL) activation, lymphokine production, motility, and adhesion.
• In natural killer (NK) cells, CD8A homodimers at the cell surface provide a survival mechanism, enabling conjugation and lysis of multiple target cells.
• CD8 alpha promotes the survival and differentiation of activated lymphocytes into memory CD8 T-cells, supporting long-term adaptive immunity.
• The protein possesses an immunoglobulin-like domain and is anchored in the cell membrane as a transmembrane glycoprotein, with key post‑translational modifications including disulfide bond formation, glycosylation, and palmitoylation.
• Subcellular localization is predominantly the cell membrane, consistent with its role in immune surveillance and cell‑mediated cytotoxicity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–186 of the feline CD8 alpha extracellular domain, ensuring recognition of the native, cell‑surface epitope; verify expression on target cells prior to flow cytometry.
• This antibody is recommended for FC applications; use appropriate isotype controls and optimize titrations for feline lymphocyte populations.
• Because CD8 alpha is expressed on both cytotoxic T cells and a subset of NK cells, consider co‑staining with lineage markers to distinguish cell types.
• For feline studies, validate cross‑reactivity with your specific sample source and blocking conditions to minimize non‑specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw material procurement, offering this well‑characterized rabbit monoclonal antibody against feline CD8 alpha to support veterinary immunology and diagnostic development. Alongside this product, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare‑target raw materials to accelerate your assay design. Connect with our team to explore how we can streamline your transition from concept to clinic.