Rabbit anti-Cat CD8 alpha Monoclonal Antibody for FC - P41688

Core Product Specifications and Parameters

Biological Background: CD8 alpha Function and Localization

• T-cell surface glycoprotein CD8 alpha chain is an integral membrane glycoprotein essential for immune responses against external and internal offenses.
• Functions as a coreceptor for MHC class I molecule:peptide complex, interacting simultaneously with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells (APCs).
• Recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling pathways that lead to lymphokine production, motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs).
• This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells.
• In NK-cells, CD8A homodimers provide a survival mechanism allowing conjugation and lysis of multiple target cells.
• Promotes the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
• Subcellular location: cell membrane.
• Keywords include adaptive immunity, disulfide bond, glycoprotein, immunoglobulin domain, lipoprotein, palmitate, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 25-186) of cat CD8 alpha, which is expected to be exposed on the cell surface for antibody access in native conditions.
• This antibody is directly conjugated with , enabling direct flow cytometry without a secondary antibody; consider titrating to determine the optimal concentration for your sample.
• For flow cytometry, maintain cells in suspension and avoid fixation protocols that may mask the epitope; validate in appropriate feline lymphocyte samples.
• CD8 alpha migrates at 32-34 kDa in SDS-PAGE due to glycosylation, consistent with its glycoprotein nature; use appropriate molecular weight markers in any complementary western blot experiments.
• Cross-reactivity has been validated for cat; for other species, examine CD8A sequence homology and test empirically if needed.
• Performance in applications beyond flow cytometry (e.g., IHC, ELISA) has not been specified and should be experimentally determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this anti-CD8 alpha antibody for immunology and T-cell research. We offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets, ensuring streamlined procurement from concept to clinic.