Rabbit Anti-CALCA Polyclonal Antibody for WB and ELISA Research Applications - P01258 / P06881

Core Product Specifications and Parameters

Biological Background: CALCA Function and Localization

• CALCA encodes a preproprotein that undergoes proteolytic processing to yield calcitonin, katacalcin, and other peptides, with alternative splicing generating multiple isoforms.
• Calcitonin is a peptide hormone secreted by thyroid C-cells; it rapidly lowers blood calcium and phosphate levels by promoting their incorporation into bone. This function is mediated by the calcitonin receptor (CALCR) and the CALCR-RAMP2 receptor complex. Related references: PMID:35324283
• Katacalcin is another potent plasma calcium-lowering peptide derived from the same precursor.
• The hormone features a disulfide bond, C-terminal amidation, and cleavage at paired basic residues, characteristic of neuroendocrine peptides.
• Subcellular localization: The preproprotein is processed through the secretory pathway, and mature peptides are secreted extracellularly.
• Key post-translational modifications and structural features include amidation, disulfide bond formation, and cleavage on paired basic residues, as indicated by UniProt keywords.
• The immunogen (amino acids 1–141) spans the full-length preproprotein, enabling potential detection of precursor and processed forms depending on antibody specificity.

Experimental Guidance and Technical Tips

• For Western blot, the antibody may detect bands corresponding to the preproprotein (~15 kDa) and/or processed forms (~13 kDa) of CALCA; validate in the relevant sample system (e.g., thyroid tissue or cell lysates).
• Consider optimizing antibody concentration and blocking conditions to minimize non-specific binding, especially when working with tissue samples.
• For ELISA, use appropriate coating buffers and validate detection parameters for the target analyte in your specific assay matrix.
• Cross-reactivity with mouse and rat has been reported; confirm reactivity in your own experimental models if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities by providing one-stop access to IVD raw materials, including validated antibody pairs and optimized polyclonal/monoclonal antibodies. This anti-CALCA antibody aids investigations into calcium metabolism, hormone signaling, and related endocrine disorders. We also offer bulk ancillary reagents, rare target sourcing, and technical services to streamline your procurement from concept to clinic.