Rabbit Anti-BGAT (ABO) Polyclonal Antibody for WB and ELISA - P16442

Core Product Specifications and Parameters

Biological Background: BGAT Function and Localization

• Belongs to the glycosyltransferase family and is the key enzyme determining the ABO blood group system. The protein exists in different allelic forms (A and B transferases) that catalyze the addition of N-acetyl-alpha-D-galactosamine (A antigen) or galactose (B antigen) to the H antigen precursor, while O alleles produce inactive protein.
• Encoded by the ABO gene, the transferase catalyzes the alpha1,3 linkage of GalNAc or Gal to the terminal galactose of all five types of naturally occurring H antigens, forming A and B blood group antigenic structures.
• Localized primarily to the Golgi stack membrane, where it functions in glycosylation; a secreted form has also been reported.
• Expressed at high levels in testis, with additional expression in pancreas, uterus, lung, and salivary gland.
• Classified as a metal-binding glycoprotein, requiring manganese as a cofactor for transferase activity.
• The protein is a single-pass type II membrane protein with a signal-anchor transmembrane helix, and undergoes proteolytic cleavage to generate a soluble form.

Experimental Guidance and Technical Tips

• The polyclonal nature may provide robust detection across species (human, mouse, rat). For Western blot, the predicted molecular weight is ~41 kDa; consider using whole cell lysates from blood cells or tissues known to express ABO transferase (e.g., testis, pancreas).
• For ELISA, validate the antibody with recombinant protein or cell lines expressing specific ABO antigens.
• Due to sequence similarity among ABO transferase alleles, the antibody may detect multiple variants; verify specificity in the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. This anti-BGAT rabbit polyclonal antibody supports ABO blood group research and diagnostic assay development. Contact us to discuss your specific procurement and technical requirements.