Rabbit Anti-ATF5 Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y2D1

Core Product Specifications and Parameters

Biological Background: ATF5 Function and Localization

• ATF5, also known as ATFx, is a cAMP-dependent transcription factor that binds CRE, ARE, and AARE elements in target gene promoters.
• It critically regulates cell survival, proliferation, and differentiation in a cell-type-dependent manner. Related references: PMID:10373550, PMID:15358120, PMID:20654631
• Promotes cell proliferation and survival by inducing EGR1 synergistically with ELK1, and upon acetylation by EP300, binds ARE to activate BCL2 and EGR1. Related references: PMID:21791614
• In liver, cooperates with NR1I3 to activate CYP2B6 transcription, represses CRE-dependent transcription to block proliferation at G2/M, and modulates IL1B signaling via NLK-mediated C/EBP activation. Related references: PMID:18332083, PMID:18701499, PMID:22528486, PMID:24379400, PMID:25512613
• Functions as a cofactor of CEBPB to promote adipocyte differentiation. Related references: PMID:24216764
• Regulates centrosome dynamics by forming a 9-foci ring scaffold around the mother centriole. Related references: PMID:26213385
• Subcellular localization includes cytoplasm, nucleus, and centrosome; broadly expressed with highest levels in liver.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region in the C-terminal half of ATF5, which may recognize full-length and certain isoforms; validate in relevant sample systems.
• This antibody is validated for WB, IHC-P, and ELISA. For WB, an expected band at ~31 kDa; consider nuclear/cytoplasmic fractionation to enrich signal.
• Cross-reactivity with mouse and rat suggests a conserved epitope; verify in each species if used for non-human samples.
• For IHC-P, antigen retrieval and dilution optimization are recommended.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw-material partner, CamelBio supplies validated antibodies and reagents for every stage from concept to clinic. This ATF5 monoclonal antibody aids research into transcriptional control, hepatic function, and centrosome biology, and we offer comprehensive antibody panels and bulk reagents to accelerate your diagnostic development.