RAB4A Rabbit pAb for WB and ELISA - P20338

Core Product Specifications and Parameters

Biological Background: Ras-related protein Rab-4A Function and Localization

• Rab-4A functions as a molecular switch cycling between inactive GDP-bound and active GTP-bound states, regulating intracellular membrane trafficking from vesicle formation to fusion. Related references: PMID:15907487 PMID:16034420
• RAB4A is involved in protein transport and vesicular traffic. Related references: PMID:29425100
• It mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling. Related references: PMID:29425100
• RAB4A acts as a regulator of platelet alpha-granule release during platelet activation and aggregation.
• Subcellular localization includes early endosome membrane, recycling endosome membrane, cytoplasm, and membrane.
• The protein features GTP-binding, hydrolase activity, magnesium and metal-binding, and undergoes methylation, phosphorylation, and prenylation.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 24 kDa; use positive controls from human, mouse, or rat cell lysates.
• Validate antibody specificity in the relevant sample system; pre-adsorption with the immunizing peptide can help confirm specificity.
• For ELISA, titrate the antibody to determine optimal working concentrations for capture or detection.
• Consider using protease inhibitors during sample preparation to avoid degradation of the small GTPase.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-RAB4A polyclonal antibody supports intracellular trafficking and protein transport research, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing.