RAB3D Rabbit pAb for WB, ELISA - O95716

Core Product Specifications and Parameters

Biological Background: RAB3D Function and Localization

• RAB3D (Ras-related protein Rab-3D) is a small GTPase belonging to the Rab family, which orchestrates intracellular membrane trafficking from vesicle formation to fusion.
• It is implicated in the regulation of insulin-stimulated exocytosis of GLUT4-containing vesicles in adipocytes, facilitating glucose transport (By similarity).
• The protein localizes to the cell membrane, consistent with its function in vesicle docking and fusion.
• RAB3D is highly expressed in granulocytes of peripheral blood and shows low constitutive expression across various hematopoietic cell lines. Related references: PMID:10023084
• Post-translational modifications, including prenylation, acetylation, and phosphorylation, regulate its membrane association and activity.
• As a GTPase, RAB3D cycles between an active GTP-bound state and an inactive GDP-bound state, a switch essential for effector recruitment and downstream signaling.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against recombinant human RAB3D (aa 1–219); consider using granulocyte or hematopoietic cell lysates as positive controls for Western blotting.
• For ELISA applications, coating with the recombinant immunogen may help establish a standard curve; validate assay conditions in the relevant sample matrix.
• Given the 24kDa predicted molecular weight, ensure adequate separation in SDS-PAGE and appropriate transfer conditions for low-molecular-weight proteins.
• Cross-reactivity with mouse RAB3D is indicated by the supplier; if working with murine samples, verify specificity through knockout or knockdown controls.

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