RAB34 Rabbit Polyclonal Antibody for WB, ELISA - P0DI83/Q9BZG1

Core Product Specifications and Parameters

Biological Background: RAB34 Function and Localization

• RAB34 belongs to the RAS oncogene family and encodes a small GTPase, with the NARR isoform generated through alternative splicing.
• The NARR isoform is characterized by nine amino-acid residue-repeats, which may facilitate specific protein interactions.
• Subcellular localization studies show that the NARR isoform is predominantly found in the nucleus and nucleolus.
• Post-translational modification analysis indicates that the protein is phosphorylated, suggesting regulatory control via phosphorylation pathways.
• The isoform has been validated at the protein level through direct protein sequencing and proteomics identification, confirming its expression.
• The molecular weight of the NARR isoform is approximately 21 kDa, as recorded in UniProt, with additional isoforms observed at 28 and 29 kDa.

Experimental Guidance and Technical Tips

• For Western blot detection, prepare whole cell lysates from human, mouse, or rat cell lines. Multiple bands corresponding to different isoforms (21, 28, and 29 kDa) may be observed. Consider using nuclear fractionation to enrich for the NARR isoform.
• In ELISA applications, use recombinant RAB34 protein as the coating antigen. Optimize coating concentration and detection parameters with appropriate negative and positive controls.
• As a polyclonal antibody, ensure lot-to-lot consistency by performing side-by-side comparisons if used across extended studies.
• Validate the antibody in your specific sample matrix, as cross-reactivity and background may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, from validated antibodies and antigens to bulk ancillary reagents, supporting diagnostic development at every stage. This anti-RAB34 antibody facilitates research into nucleolar functions and alternative splicing, and we can assist with customized sourcing for rare targets. Expedite your assay development by partnering with CamelBio to streamline procurement and ensure consistent supply.