PYGB Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P11216

Core Product Specifications and Parameters

Biological Background: PYGB Function and Localization

• PYGB, also known as GPBB, is the brain-specific isoform of glycogen phosphorylase, a key enzyme in glycogen metabolism.
• Glycogen phosphorylase catalyzes the rate-limiting step of glycogenolysis, mobilizing glucose from glycogen stores (PubMed:27402852). Related references: PMID:27402852
• PYGB is an allosteric enzyme crucial for carbohydrate metabolism, regulated by both covalent modification and allosteric effectors (PubMed:3346228). Related references: PMID:3346228
• Conserved catalytic and structural features are shared among all phosphorylases, including cofactor binding for pyridoxal phosphate (PubMed:3346228). Related references: PMID:3346228
• Post-translational modifications such as phosphorylation and acetylation modulate PYGB activity and cellular signaling.
• PYGB’s brain-specific expression underscores its role in neural energy homeostasis and potential involvement in neurological disorders.

Experimental Guidance and Technical Tips

• For Western blotting, an expected band at ~97 kDa should be observed; using lysates from human, mouse, or rat brain or muscle tissues is recommended.
• Due to the polyclonal nature, validate specificity in your sample matrix and consider using optimized blocking and antibody dilution conditions.
• For IF/ICC, fixation and permeabilization methods may require optimization; test methanol or paraformaldehyde fixation and Triton X-100 permeabilization.
• In immunoprecipitation assays, protein A/G bead compatibility should be assessed, and appropriate controls for non-specific binding are advisable.
• ELISA applications may be feasible, but direct coating or sandwich formats should be validated with known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our expertise in carbohydrate metabolism and brain-specific proteins supports efficient sourcing for assay development. Contact us to discuss custom PYGB antibody solutions or to explore rare target raw-material procurement for your next project.