PUMA Rabbit pAb for WB - Q96PG8/Q9BXH1

Core Product Specifications and Parameters

Biological Background: PUMA Function and Localization

• PUMA (p53 up-regulated modulator of apoptosis), also known as Bcl-2-binding component 3 (BBC3) or JFY-1, is encoded by the BBC3 gene in humans (Gene ID: 27113).
• The protein undergoes alternative splicing, producing multiple isoforms with distinct molecular weights; the canonical isoform (UniProt: Q96PG8) has a calculated mass of approximately 26.5 kDa.
• PUMA is classified within the Bcl-2 protein family, characterized by its ability to bind Bcl-2.
• Functional annotation indicates that PUMA does not affect cell growth under certain experimental conditions (UniProt).
• This protein is included in the UniProt reference proteome set and has been identified through proteomics approaches (keyword: Proteomics identification).
• Additional keywords include alternative splicing, reflecting the diversity of transcribed variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–131 of human PUMA (NP_001120713.1), a region likely conserved across the recognized isoforms, which may result in detection of multiple bands (10, 14, 20, and 26 kDa) in Western blotting.
• For Western blot, consider using whole-cell lysates from human, mouse, or rat cell lines expressing PUMA; optimize primary antibody dilutions and blocking conditions empirically.
• For IHC-P, formalin-fixed paraffin-embedded tissues should be evaluated; antigen retrieval methods (e.g., citrate or EDTA buffer) may be necessary.
• For IF/ICC, fixation and permeabilization protocols should be validated in the cell type of interest; a nuclear or cytoplasmic localization may be observed depending on cellular context.
• For ELISA, the antibody can serve as a capture or detection reagent; pairing with a validated detection antibody or peptide competition may improve specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibodies like this anti-PUMA polyclonal antibody for apoptosis-related research. Our one-stop sourcing platform covers monoclonal and polyclonal antibodies, antigen pairs, and ancillary reagents, supporting your project from concept to clinic. For specialized targets or bulk requirements, please contact our team for customized solutions.