PUMA Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BXH1

Core Product Specifications and Parameters

Biological Background: PUMA Function and Localization

• PUMA (p53 up-regulated modulator of apoptosis, also known as Bcl-2-binding component 3, JFY-1) is encoded by the BBC3 gene and functions as an essential mediator of both p53/TP53-dependent and -independent apoptosis.
  Related references:
  PMID:11463391
  PMID:23340338

• It promotes apoptosis by inducing partial unfolding of BCL2L1 and dissociating it from p53/TP53, thereby releasing p53/TP53 to trigger cell death.
  Related references:
  PMID:23340338

• PUMA is localized to the mitochondrion, consistent with its role in the intrinsic apoptotic pathway.

• It is ubiquitously expressed across human tissues, highlighting its broad regulatory role in apoptosis.

• The protein undergoes alternative splicing and phosphorylation, as indicated by UniProt keywords, which may modulate its pro-apoptotic activity.

• PUMA also regulates ER stress-induced neuronal apoptosis, as suggested by similarity data.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band around 18 kDa is expected based on the product's protein weight specification; consider using appropriate controls to confirm specificity.
• In IHC-P and IF/ICC, optimization of antigen retrieval and blocking steps may be necessary for robust detection in human, mouse, or rat tissues.
• The immunogen corresponds to a synthetic peptide spanning amino acids 100–193, which may be useful for peptide blocking experiments to validate antibody binding.
• As a monoclonal antibody, it offers consistent lot-to-lot performance; nevertheless, each new batch should be validated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For apoptosis-focused studies targeting PUMA, we provide validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our streamlined supply chain ensures reliable access to high-quality raw materials for your assay development needs.