PTPRO Rabbit Polyclonal Antibody for WB and ELISA - Q16827

Core Product Specifications and Parameters

Biological Background: PTPRO Function and Localization

• PTPRO encodes a receptor-type tyrosine-protein phosphatase that removes phosphate groups from phosphotyrosine residues, acting as a key regulator of signaling pathways.
• It exists in multiple isoforms due to alternative splicing, which may exhibit distinct tissue distributions and functional roles.
• The protein plays a critical role in kidney physiology by modulating podocyte structure and function, influencing the glomerular pressure–filtration rate balance.
• PTPRO is a transmembrane protein localized to the plasma membrane, featuring an extracellular domain, a single-pass transmembrane helix, and an intracellular phosphatase domain.
• It is highly expressed in the glomerulus of the kidney, with additional detection in brain, lung, and placenta.
• Post-translational modifications include glycosylation and phosphorylation, which may regulate its activity, stability, or localization.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein encompassing the N-terminal extracellular region (aa 30–300) of human PTPRO; the antibody may recognize linear epitopes in that region and could detect both glycosylated and unglycosylated forms.
• For Western blot, expect a predominant band near 138 kDa, although alternative splicing and post-translational modifications may produce additional bands; use appropriate tissue or cell lysates known to express PTPRO (e.g., kidney, brain).
• When developing an ELISA, consider pairing this polyclonal antibody with a monoclonal capture antibody, or use the recombinant immunogen as a standard for quantitative assays.
• Cross-reactivity with mouse PTPRO is reported; validate in the relevant mouse model system and check sequence alignment of the immunogen region to ensure consistent reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supply partner, offering integrated sourcing solutions for high-quality antibodies, antigens, and ancillary reagents. This anti-PTPRO antibody supports nephrology and cell signaling research, and CamelBio can provide matching antigen, detection antibody pairs, or custom bulk production to streamline your diagnostic development. Our portfolio covers rare targets, ensuring you stay focused on validation and clinical translation.