PTPN5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P54829

Core Product Specifications and Parameters

Biological Background: PTPN5 Function and Localization

• PTPN5, also known as STEP (Striatum-enriched protein-tyrosine phosphatase), is a neural-specific non-receptor type tyrosine phosphatase.
• It plays a critical role in synaptic plasticity and neuronal cell survival by regulating the activity of MAPKs, Src family kinases, and NMDA receptors.
• The gene undergoes alternative splicing, producing multiple isoforms that may differ in function and localization.
• The protein is primarily localized to the endoplasmic reticulum membrane, where it may interact with downstream signaling molecules.
• PTPN5 is post-translationally modified by phosphorylation, influencing its catalytic activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 190-307), suggesting the antibody may recognize multiple splice variants if this region is conserved. Validate in relevant cell lines (e.g., neuronal cultures) that express PTPN5.
• For Western blotting, a band at approximately 64 kDa is expected. Use optimized blocking and detection steps for rabbit polyclonal antibodies.
• For IF/ICC, fixation and permeabilization conditions should be validated to preserve the endoplasmic reticulum membrane localization of PTPN5. Include appropriate controls.
• ELISA performance can be influenced by the recombinant protein coating concentration; titration experiments are recommended.
• Cross-reactivity with mouse and rat has been reported; however, tissue-specific expression in brain regions should be confirmed experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, providing diagnostic manufacturers and research institutes with high-quality antibodies, recombinant proteins, and ancillary reagents. This PTPN5 polyclonal antibody is part of our growing neuroscience portfolio, supporting research into synaptic plasticity and neurological disorders. For validated antibody pairs, bulk orders, or rare target sourcing, contact our dedicated team to streamline your workflow from concept to clinic.