PTPN22 Rabbit pAb for WB, IF/ICC, ELISA - Q9Y2R2

Core Product Specifications and Parameters

Biological Background: PTPN22 Function and Localization

• PTPN22 (also known as LYP, PEP) is a non-receptor tyrosine phosphatase primarily expressed in immune cells.
• Acts as a negative regulator of TCR signaling by dephosphorylating Src kinases LCK and FYN, ZAP70, VAV, VCP, CBL, and SKAP2.
  Related references: PMID:16461343, PMID:18056643, PMID:21719704
• Positively regulates TLR-induced type I interferon production, contributing to antiviral innate immunity.
  Related references: PMID:23871208
• Regulates NOD2-induced inflammatory signaling and activates NLRP3 inflammasome via dephosphorylation of NLRP3.
  Related references: PMID:23991106, PMID:27043286
• Expressed predominantly in immune cells including T-cells, B-cells, NK cells, monocytes, dendritic cells, and neutrophils, with isoform 1 being the major form in lymphocytes.
  Related references: PMID:15208781
• Localizes to the cytoplasm and is genetically associated with autoimmune diseases such as type 1 diabetes and systemic lupus erythematosus.
• Undergoes alternative splicing yielding multiple isoforms with potentially specialized functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-90) of human PTPN22; the antibody may detect full-length protein and isoforms containing this region. Validate in your target species.
• For Western blot, expect a band around 92 kDa; use appropriate negative and positive controls.
• For immunofluorescence/ICC, anticipate cytoplasmic localization. Perform fixation and permeabilization optimization.
• Cross-reactivity with mouse and rat PTPN22 has been suggested; confirm specificity with species-appropriate controls and consider potential background signals.
• For ELISA, titrate the antibody and pair with an appropriate detection system; pre-validate with recombinant protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions from early development to clinical application. Our PTPN22 antibody facilitates research into autoimmune disorders and immune signaling, backed by CamelBio's extensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. Whether you require custom antibody development, bulk raw material supply, or difficult-to-source targets, CamelBio ensures reliable access, technical consultation, and seamless integration into your workflow.