PTBP1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26599

Core Product Specifications and Parameters

Biological Background: PTBP1 Function and Localization

• PTBP1, also known as hnRNP I or 57 kDa RNA-binding protein, is a member of the heterogeneous nuclear ribonucleoprotein family.
• It plays a key role in pre-mRNA splicing by binding polypyrimidine tracts and regulating alternative splicing events, including exon skipping in its own pre-mRNA during muscle cell differentiation.
• PTBP1 represses the splicing of MAPT/Tau exon 10. Related references: PMID:15009664
• It binds to the polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, antagonizing TIA1. Related references: PMID:14966131
• PTBP1 regulates pyruvate kinase PKM splicing by repressing exon 9 inclusion, leading to production of the PKM M2 isoform. Related references: PMID:20010808
• Upon picornavirus infection, PTBP1 binds to the viral IRES and stimulates IRES-mediated translation. Related references: PMID:21518806
• The protein is predominantly localized in the nucleus and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N-terminal sequence (aa 1-100) of human PTBP1; confirm antibody reactivity against full-length protein in your experimental system.
• For Western blotting, a band around 60 kDa is expected; optimize loading and antibody dilution for your samples.
• In IHC-P, antigen retrieval may be required; validate with appropriate positive control tissues.
• For IF/ICC, consider using 4% PFA fixation and permeabilization with Triton X-100.
• ELISA applications may require optimization of coating antigen and antibody concentration; test under your assay conditions.
• Cross-reactivity with human and mouse is reported; verify reactivity in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers with one-stop access to high-quality IVD raw materials like this PTBP1 monoclonal antibody for RNA biology research. We provide validated antibody pairs, bulk reagents, and rare target sourcing to accelerate your assay development. Leverage our integrated supply chain to bridge the gap from concept to clinic efficiently.