PSMD8 Rabbit pAb for WB, IF/ICC, ELISA - P48556

Core Product Specifications and Parameters

Biological Background: PSMD8 Function and Localization

• PSMD8, also known as 26S proteasome non-ATPase regulatory subunit 8 (p31, S14, RPN12), is a subunit of the 26S proteasome regulatory particle.
• The 26S proteasome is a large multi-subunit complex responsible for ATP-dependent degradation of ubiquitinated proteins, maintaining cellular protein homeostasis.
• PSMD8 is a non-ATPase regulatory subunit that contributes to substrate recognition and translocation into the proteolytic core particle.
• The proteasome, including PSMD8, plays critical roles in cell cycle progression, apoptosis, and DNA damage repair by removing regulatory proteins.
• Post-translational modifications: According to UniProt, PSMD8 undergoes phosphorylation and isopeptide bond formation, and is also subject to ubiquitin-like conjugation.
• It is widely expressed and serves as a reference proteome component in human proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 101-350, the central region of PSMD8. This design likely generates antibodies recognizing both native and denatured forms, suitable for multiple applications.
• For Western blot, a band at approximately 40 kDa is expected; consider using appropriate loading controls and optimizing antibody dilution.
• In IF/ICC, fixation and permeabilization conditions may influence epitope accessibility; titrate the antibody in the relevant cell line.
• ELISA can be used for quantifying PSMD8 in cell or tissue lysates; pairing with a validated capture antibody may improve sensitivity.
• The antibody shows cross-reactivity with human, mouse, and rat; validate in the corresponding species for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This anti-PSMD8 polyclonal antibody supports proteasome research and ubiquitin-proteasome pathway studies. Beyond individual antibodies, CamelBio offers custom bulk production and sourcing of rare targets, providing a streamlined bridge from concept to clinical application.