PSMD14 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00487

Core Product Specifications and Parameters

Biological Background: PSMD14 Function and Localization

• PSMD14 is a metalloprotease subunit of the 26S proteasome responsible for ATP-dependent degradation of ubiquitinated proteins, playing a key role in protein homeostasis. Related references: PMID:9374539, PMID:1317798
• It specifically cleaves 'Lys-63'-linked polyubiquitin chains, facilitating deubiquitination of substrates within the proteasome. Related references: PMID:22909820
• PSMD14 participates in DNA double-strand break repair by regulating non-homologous end joining (NHEJ) through cleavage of 'Lys-63'-linked polyubiquitin, which promotes chromatin retention of JMJD2A/KDM4A and restricts TP53BP1 accumulation. Related references: PMID:22909820
• It also promotes homologous recombination repair by enhancing RAD51 loading at damage sites. Related references: PMID:22909820
• PSMD14 regulates macroautophagy via its deubiquitinating activity on K63-linked ubiquitin chains, ensuring Golgi-to-ER retrograde transport and preventing retention of autophagy proteins at the Golgi. Related references: PMID:32210007
• Widely expressed with highest levels in heart and skeletal muscle.
• Keywords: Metalloprotease, proteasome, DNA repair, autophagy, zinc-binding; phosphorylated.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant fragment (amino acids 112-210) of human PSMD14, which spans the central region of the protein; it may recognize both native and denatured forms.
• For western blotting, a band at approximately 35kDa is expected; verify specificity using appropriate controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval and permeabilization steps to optimize staining.
• This monoclonal antibody can be utilized in ELISA; validate its performance as a capture or detection reagent in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with convenient access to high-quality IVD raw materials like this PSMD14 antibody, ideal for studying the ubiquitin-proteasome system, DNA repair, and autophagy. We offer bulk and customized supplies of validated antibodies, ancillary reagents, and can source challenging targets to streamline your assay development and manufacturing pipelines. Contact us today to explore how our integrated raw-material solutions can support your project from concept to clinic.