PSMD12 Rabbit Polyclonal Antibody for WB, ELISA - O00232

Core Product Specifications and Parameters

Biological Background: PSMD12 Function and Localization

• PSMD12, also known as Rpn5 or p55, is a component of the 26S proteasome, a large multi-subunit complex responsible for ATP-dependent degradation of ubiquitinated proteins.
• It plays a critical role in maintaining protein homeostasis by eliminating misfolded, damaged, or unneeded proteins.
• The 26S proteasome participates in diverse cellular processes including cell cycle progression, apoptosis, and DNA damage repair.
• PSMD12 belongs to the proteasome non-ATPase regulatory subunit family and contributes to the lid subcomplex of the 19S regulatory particle.
• Post-translational modifications include acetylation and ubiquitin-like conjugation, which may regulate its function or interactions.
• The protein has been identified through proteomics and direct protein sequencing, and its three-dimensional structure has been resolved.
• Mutations in PSMD12 are associated with intellectual disability, and alternative splicing generates multiple isoforms.
• The calculated molecular weight is approximately 53 kDa, consistent with both UniProt and experimental data.

Experimental Guidance and Technical Tips

• For Western blotting, use standard protocols with appropriate loading controls; validate antibody specificity in your sample system by including positive and negative controls.
• For ELISA, consider direct or sandwich formats; optimize coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratio.
• The immunogen corresponds to the internal region (aa 197–456), so the antibody may detect full-length and some truncated forms; verify reactivity in your specific application.
• Indicated cross-reactivity with human, mouse, and rat suggests conservation of the epitope; for other species, check sequence homology in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like anti-PSMD12, and bulk ancillary reagents. With deep expertise in the ubiquitin-proteasome system, we support diagnostic manufacturers and research labs in sourcing rare-target reagents efficiently. Reach out to leverage our one-stop procurement services for your proteasome or protein degradation research needs.