PSMC3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17980

Core Product Specifications and Parameters

Biological Background: PSMC3 Function and Localization

• PSMC3, also known as 26S proteasome regulatory subunit 6A, RPT5, TBP1, or proteasome subunit P50, is a core component of the 26S proteasome.
• It belongs to the AAA ATPase family and is essential for the ATP-dependent degradation of ubiquitinated proteins, thereby maintaining cellular protein homeostasis.
• As part of the 19S regulatory particle, PSMC3 forms a heterohexameric ring that unfolds substrates and translocates them into the 20S core for proteolysis.
• The protein shuttles between the cytoplasm and nucleus, consistent with its role in ubiquitin-proteasome system (UPS) function across cellular compartments.
• UniProt annotations indicate post-translational modifications including acetylation and phosphorylation, and involvement in host-virus interactions via its Tat-binding property.
• It is implicated in processes such as cell cycle progression, apoptosis, DNA damage repair, and has been linked to disorders like cataract, deafness, intellectual disability, and neuropathy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–180) of human PSMC3. Verify that the antibody recognizes the expected epitope in your experimental system.
• For Western blot, the predicted molecular weight is approximately 49 kDa; use appropriate positive and negative controls to confirm specificity.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval conditions and antibody dilution based on tissue type.
• In ELISA, the recombinant protein used as immunogen can serve as a positive control to assess antibody reactivity.
• Cross-reactivity with mouse and rat PSMC3 is indicated; validation in relevant species and tissues is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD raw material sourcing, supplying high-quality antibodies, proteins, and ancillary reagents to diagnostic manufacturers and research laboratories worldwide. This anti-PSMC3 antibody supports proteasome and ubiquitin-proteasome pathway investigations, and we offer complementary raw materials—from validated antibody pairs to bulk monoclonals—for streamlined assay development. From target selection to clinical translation, trust CamelBio to bridge your supply needs with scientific reliability.